Using these tips typically follows a simple four-step process:
Using a high-organic solvent to release the purified protein directly onto a MALDI plate or into a fresh tube. Real-World Application: Mass Spectrometry
In the world of proteomics, the quality of your mass spectrometry data is only as good as your sample preparation. Contaminants like salts, buffers, and detergents can easily drown out the signals of the very proteins you’re trying to study. This is where become indispensable. What are C4 Zip-tips? Using these tips typically follows a simple four-step
Produced by Millipore , C4 Zip-tips are 10 µL pipette tips containing a specialized C4 (butyl) stationary phase resin. Unlike the more common C18 tips used for peptides, the C4 resin is optimized for larger, more hydrophobic molecules like intact proteins and large polypeptides. Why C4 over C18?
While C18 is the "gold standard" for peptide mapping, it can bind proteins so tightly that they become nearly impossible to elute. The shorter carbon chain of the C4 resin offers: This is where become indispensable
Aspirating and dispensing the protein sample multiple times to maximize binding.
Preparing the resin with a wetting solution (e.g., 50:50 acetonitrile in water). Unlike the more common C18 tips used for
Rinsing with a buffer like 0.1% trifluoroacetic acid (TFA).