Electrophoresis ⭐ Best

The standard method for separating DNA fragments, often used after a PCR (Polymerase Chain Reaction) to verify results.

Molecules are loaded into a porous gel, typically made of agarose (for large DNA fragments) or polyacrylamide (for smaller DNA or proteins). This gel acts as a molecular sieve. Electrophoresis

Uses narrow tubes instead of gels, allowing for high-speed separation and automation, commonly used in modern DNA sequencing. Real-World Applications The standard method for separating DNA fragments, often

The process relies on the principle that many biological molecules possess an electrical charge. When placed in an electric field, these molecules migrate toward the electrode with the opposite charge. allowing for high-speed separation and automation

DNA profiling (DNA fingerprinting) uses electrophoresis to compare crime scene samples with suspect DNA.