Plant Propagation By Tissue Culture -

: Individual shoots are transferred to a rooting medium, usually containing auxins (like IBA or NAA), to develop a healthy root system.

To succeed, tissue culture requires a highly controlled environment: Plant Propagation by Tissue Culture

Plant tissue culture, or , is a laboratory-based method of cloning plants by growing cells, tissues, or organs in a sterile, nutrient-rich environment. It allows for the rapid production of thousands of identical, disease-free plants from a single tiny piece of tissue, regardless of the season. Core Stages of Tissue Culture The process typically follows four essential steps: : Individual shoots are transferred to a rooting

: The explant is moved to a medium enriched with hormones (often cytokinins like BAP) to stimulate the growth of multiple new shoots. or organs in a sterile