The technique operates on the principle of . When a molecule absorbs UV or visible light, its electrons move from a low-energy ground state to a higher-energy excited state. The Beer-Lambert Law
UV‑Vis Spectroscopy: Principles, Strengths and Applications UV-VIS Spectroscopy and Its Applications
(Molar Absorptivity): A constant specific to the substance and wavelength. (Path Length): Typically 1 cm, using a standard cuvette. Essential Components The technique operates on the principle of